Journal: Journal of Functional Foods

Article Title: Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds

doi: 10.1016/j.jff.2021.104786

Figure Lengend Snippet: Fig. 4. γ-conglutin peptides stimulate mTOR downstream pathway in HSMM: Western blot analysis of (A) mammalian target of rapamycin (mTOR) and (B) AKT (protein kinase B) (C) glycogen synthase kinase-3β (GSK3β) (D) 70 KDa ribosomal protein S6 kinase (70S6K) (E) ribosomal protein S6 (S6) and (F) extracellular signal-regulated kinase (ERK1/2) in HSMM cell lysate and ratio of their band intensities (phosphorylated (p)/total (t) or phosphorylated (p)/GAPDH) in presence of vehicle (control referred to as ‘C’), positive control – insulin (100 nM) (referred as ‘I’) or γ-conglutin peptides (200–2 µg/mL). The ratio of band intensities is reported as values normalised with control. Values are mean ± standard deviation of three or more independent experiments; ****p ≤0.0001, ***p ≤0.001, **p ≤0.01 and *p ≤0.05 are significantly different as compared to control. Immunoblots shown are representative of three or more independent experiments.

Article Snippet: Polyclonal goat antirabbit immunoglobulins/HRP from Dako (Glostrup, Denmark) and protease/phosphatase inhibitor cocktail (100X) and primary rabbit mAb (p-mTOR (Ser 2448; #2971S), p-AKT (Ser 473, #9271S), p-S6 (Ser235/ 236, #4858S), p-GSK3B (Ser9; 9336S), p-ERK1/2 (#4370), GAPDH (#D16H11), mTOR (#2972S), AKT (#9272S), S6 (#2217S), GSK3B (#9315S), ERK1/2 (#4695)) were obtained from Cell Signaling Technology (Massachusetts, USA).

Techniques: Western Blot, Control, Positive Control, Standard Deviation

Journal: Cell reports

Article Title: HRS mediates tumor immune evasion by regulating proteostasis-associated interferon pathway activation

doi: 10.1016/j.celrep.2023.113352

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-GAPDH (clone D16H11) , Cell Signaling Technology , Cat#5174; RRID:AB_10622025.

Techniques: Virus, Recombinant, SYBR Green Assay, Staining, shRNA, Plasmid Preparation, Software, CRISPR

Journal: iScience

Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism

doi: 10.1016/j.isci.2024.109075

Figure Lengend Snippet:

Article Snippet: GAPDH (D16H11) XP Rabbit mAb , Cell Signaling Technology , Cat#5174; RRID: AB_10622025.

Techniques: Recombinant, Glutathione Assay, Dehydrogenase Assay, Reverse Transcription, Bicinchoninic Acid Protein Assay, Software

Journal: Immunity

Article Title: Interferon-independent activities of mammalian STING mediate antiviral response and tumor immune evasion

doi: 10.1016/j.immuni.2020.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb Cell signaling Cat#5483 TBK1/NAK (D1B4) Rabbit mAb Cell signaling Cat#3504 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell signaling Cat #4947 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb Cell signaling Cat#3033 NF-κB p65 (D14E12) XP® Rabbit mAb Cell signaling Cat#8242 STING (D1V5L) Rabbit mAb (Rodent Preferred) Cell signaling Cat#50494 GAPDH (D16H11) XP® Rabbit mAb Cell signaling Cat#5174 LC3B Antibody Novus Cat#NB100–2220 PE anti-mouse CD3 Antibody Biolegend Cat#100206 FITC anti-mouse CD8a Antibody Biolegend Cat#100706 APC anti-mouse CD45 Antibody Biolegend Cat#103112 PE/Dazzle™ 594 anti-mouse IFN-γ Antibody Biolegend Cat#505845 FITC anti-mouse TNF-α Antibody Biolegend Cat#506304 PE anti-mouse Perforin Antibody Biolegend Cat#154305 APC-Granzyme B Invitrogen Cat#17-8898-80 CD3e Monoclonal Antibody (145–2C11) Invitrogen Cat#16-0031-81 CD28 Monoclonal Antibody (37.51) Invitrogen Cat#16-0281-82 Purified anti-mouse IFN-β Antibody BioLegend Cat#519202 Biotin anti-mouse IFN-β Antibody BioLegend Cat#508105 Chemicals, and Recombinant Proteins 2’3’-cGAMP Invivogen Cat#tlrl-nacga23–02 3’3’-cGAMP Invivogen Cat#tlrl-nacga 2’3’-c-di-AM(PS)2 (Rp,Rp) Invivogen Cat#tlrl-nacda2r-01 DMXAA Invivogen Cat#tlrl-dmx 9-Oxo-10(9H)-acridineacetic acid (CMA) Sigma Cat#17927 CellTrace™ CFSE Cell Proliferation Kit Invivogen Cat# {"type":"entrez-nucleotide","attrs":{"text":"C34554","term_id":"2370695","term_text":"C34554"}} C34554 Annexin V Apoptosis Detection Kit Biolegend Cat#640914 DNeasy Blood & Tissue Kits QIAGEN Cat#69506 Protease Inhibitor Cocktail Roche Cat#11873580001 Lipofectamine 2000 ThermoFisher scientific Cat#11668019 TRI Reagent Sigma Cat#T9424 Annexin V Binding Buffer Biolegend Cat#422201 RIPA Lysis Buffer Millipore Cat#20–188 ACK Lysing Buffer ThermoFisher scientific Cat#A1049201 Pierce™ BCA Protein Assay Kit ThermoFisher scientific Cat#23225 Polybrene Sigma Cat#TR-1003 DNase I Sigma Cat#11284932001 Collagenase Sigma Cat#C5138 iScript™ Reverse Transcription Supermix Bio-Rad Cat#1708840 iTaq™ Universal SYBR® Green Supermix Bio-Rad Cat#1725120 Naive CD8a+ T Cell Isolation Kit Miltenyi Biotec Cat#130-096-543 Pan T Cell Isolation Kit II Miltenyi Biotec Cat#130-095-130 Dual-Luciferase® Reporter Assay System Promega Cat#E1960 Recombinant Murine IL-2 PEPROTECH Cat#212–12 C-176 STING inhibitor BioVision Cat#B2341 Fixation/Permeabilization Solution Kit BD Cat#554714 HRP Avidin BioLegend Cat#405103 Recombinant Mouse IFN-β (ELISA Std.)

Techniques: Purification, Recombinant, Protease Inhibitor, Binding Assay, Lysis, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Cell Isolation, Reporter Assay, Avidin-Biotin Assay, Enzyme-linked Immunosorbent Assay, Staining, Software